Sample submission

The sample submission form can be downloaded here.

CMG UZ Brussel users can also retrieve the latest version from iProva ('Sample Submission Form BRIGHTcore')

 

Using the form is obligatory to submit RESEARCH AND DIAGNOSTIC samples.

 

Description on how to submit samples

  • The first step is different for RESEARCH and DIAGNOSTIC samples:

    • SUBMITTING RESEARCH SAMPLES: Under ‘CHOOSE FORM SETTINGS’ click the button ‘Research submission’. The form is now automatically adapted to the ‘RESEARCH’ setting (orange colors)

    • SUBMITTING DIAGNOSTIC SAMPLES: Under ‘CHOOSE FORM SETTINGS’ click the button ‘Diagnostic submission’. The form is now automatically adapted to the ‘DIAGNOSTIC’ setting (green colors)

 

  • The other steps are common for RESEARCH and DIAGNOSTIC samples:

    • Fill in all the required fields under CONTACT AND BILLING DETAILS (marked with ‘*’)

    • Fill in all the required fields under ASSAY DETAILS. When specifying the assay details they are automatically applied to all samples specified in the ‘SAMPLE FORM’. However, in the SAMPLE FORM you have the opportunity to specify the assay details for each sample separately. BRIGHTcore prefers that one form is submitted for each different assay type.

    • Fill in the details for each sample under SAMPLE FORM. The columns marked with ‘*’ should all contain information. The other columns are optional.

    • Click on the ‘SAVE AND SEND’ button at the top of the document. This will generate a unique ‘experiment name’ that contains the current date, the assay type and the responsible scientist. Furthermore the document will be saved on the user’s desktop. A Microsoft Outlook window will open automatically through which you can send the sample sheet to info@brightcore.be

Important information regarding sample quantification

  • All DIAGNOSTIC and RESEARCH DNA samples should be quantified fluorometrically and not spectrophotometrically.

  • RNA samples can be quantified either fluorometrically or spectrophotometrically.

  • The minimum requirements for sample amount and concentration:

    • Amount:

      • DNA : at least 2 µg (determined fluorometrically, eg. Qubit)

      • RNA : at least 200 ng (for high input assay) or lower (min. 10 pg) for low input assays

    • Concentration:

      • DNA : should exceed or be equal to 30 ng/µl 

      • RNA : should exceed or be equal to 2 ng/µl (for high output assay), for low input assays min. of 10 pg in 7 µl is allowed

    • DNA/RNA should not be degraded

 

FAQ: Which buffer should we use to dilute our DNA samples?

Important information regarding sample labeling

  • If samples are delivered in a plate, please label plate with the "Name of experiment" & indicate the orientation of samples in plate.

  • If individual tubes per sample are provided to BRIGHTcore, please label all tubes with the "Sample no.", "Unique sample ID" and "Name of Experiment"

  • Label tubes or plates with a printed label

Important information regarding sample delivery and take-out

  • DIAGNOSTIC SAMPLES: Samples can be put in the ‘stalen voor NGS – IN’ drawer of fridge 000012980 in the DNA PRE-PCR room (A05.A.113) of the Center for Medical Genetics – UZ Brussel. The responsible scientist will be informed when the data is available. Samples will be put in the ‘stalen voor NGS – OUT’ drawer of that same fridge.

  • RESEARCH SAMPLES: When samples are delivered, contact Sarah Peeters or Annick Callaerts. They will put the samples on the correct location, since different assay types might have to be stored in different places.

 

FAQ: Where should I send my samples to?

 

FAQ: Should I send my samples frozen?

 

FAQ: Can we get the leftovers of our (precious) samples back?

Important information regarding turn-around-time

  • The date mentioned in the experiment name and the name of the file represents the start of the 2 MONTHS TAT.

  • BRIGHTcore will contact the responsible scientist if the TAT will be exceeded.

  • During these 2 months, information regarding the sample and analysis status can be asked by sending an e-mail to info@brightcore.be. Asking such questions should be an exception rather than a rule.

BRIGHTcore / T +32 (0)2 477 64 79 / F +32 (0)2 477 68 59 / © 2015 by BRIGHTcore

Photography / Bart Moens / http://www.fotobart.be

Content & illustrations / Ben Caljon