GENE PANELS
STHT - LYMFOID
Sample types
We aim to keep the list with sample types updated. However, if you believe we offer this gene panel on other sample types, or if you have a very specific sample type you'd like to evaluate : please contact the corresponding persons for either diagnostic or research purposes.
* Microtube can be a either a cryovial or eppendorf tube (0,5 ; 1,5 or 2 ml). Please consider the quality of the tube used (nuclease free ; free of RNA/DNA ; sterile ; LoBind) according to application needed.
Blood > DNA
EDTA tube
10 ml (min. 3 ml)
Room temperature
DNA from various tissues > DNA
Microtube* or 96 well plate
See test details/specifications
Room temperature
FFPE block of various tissues > DNA
Paraffin block (in cassette)
Representative block ; Tumor load preferentially >10% ; To be processed by our pathology department (microtome section needed)
Room temperature
FFPE section of various tissues > DNA
Microtube*
Min. 1x 10 µm section (up to 2 mm³ tissue) ; Tumor load preferentially >10%
Room temperature
Tissue of various origins > DNA
Cryovial containing stabilizer (eg. AllProtect) or tissue homogenized on lysis buffer
Application/tissue specific (contact us)
Dry ice
Extra requirements
Absorbance ratio A260/A230 - Minimum
DNA from various tissues
Minimum: 1,5
Lower values are indicative for indicative for EDTA, carbohydrate, chaotropic salts (and also protein) contamination
Absorbance ratio A260/A280 - Minimum
DNA from various tissues
Minimum: 1,5
Lower values are indicative for indicative for protein, phenol or other aromatic compound contamination
Elution buffer : nuclease free water, Tris-HCl or TE
DNA from various tissues
:
Please use nuclease free elution buffers, however this assay is compatible with either water, Tris-HCl as TE.
Minimum DNA concentration
DNA from various tissues
Minimum: 6 ng/µl
A minimum concentration of 6 ng/µl is required, however succesfull experiments are possible below this limit. Low concentrated samples are vacuum concentrated to increase library prep success rate.
Minimum DNA quantity
DNA from various tissues
Minimum: 10 ng
Below 10 ng input, success rate is very low. Please provide us with as much as material as possible (300 ng preferred).
Deliverables
Below, you can find the type of data files that can be retrieved for this test.
If you require more information or need a more custom output, please consult our Bioinformatics page.
Called variants - vcf file
Text file containing all detected variants as compared to the relevant reference genome
Diagnostic / RUO
Clinical report
Diagnostic report generated by Clinical Geneticist/ Pathologist / Hematologist
Diagnostic
Raw data - bam file
Binary data file containing the aligned reads
RUO (on request)
Run folder
The complete run folder can be transferred if it concerns a run reservation (private run)
RUO (on request)
Costs
For Diagnostic testing
When requested through your General Practitioner / Clinical Geneticist / Pathologist / Hematologist, costs will be covered through your health insurance. The costs involved will be those for your consultation and/or 'remgeld'.
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For Research testing
Below, you can find indicative prices (excl. VAT) for all potential options you'd require to accomplish your project. If you are interested, please contact us to receive a tailored quotation.
Update pending
Our prices are being updated. More info soon.
Documents & certificates
141-MED (UZ Brussel)
Plan experiment
For Diagnostics
This test is mainly performed as a diagnostic assay. In this case, please contact your General Practitioner, Pathologist or Clinical Geneticist (Centre for Medical Genetics of choice) to get the diagnosis started.
For Research
This test can also be applied in context of research. For this, please get approval from your local ethical committee and/or make proper arrangements with your Biobank.
Please follow the steps below to start up an experiment with us.
Get in touch (ONLY FOR NEW PROJECTS)
Explain your project, so we can assist you to find the best solution.
Expect questions like :
What type of sample would you like to analyze?
Which elution buffer did you use?
What are the expected concentrations of your samples?
How many samples would you like to process?
How fast do you need the results (TAT) and/or do we need to expedite?
If you deviate from our sample types, are there test samples available?
Are our standard deliverables ok?
...
1
Request quotation
Once you know which experiment you want to set up, fill out the 'Quote request' form, with the essential details of your project :
Coordinates of the person to whom to address the quotation
Which type of test you'd like to set up
Do you require a specific deliverable?
Do we need to expedite (comes at extra cost)?
...
2
Submit your samples
Once you agree with our terms (quote / TAT / terms & conditions) :
Fill out our sample submission form
Send it via email : contact us
Print out the form and include it with your samples
Send your samples to us
Label your tubes/plates correctly : see details here
Adhere to the corresponding transport conditions listed under Sample types
Indicate if we can discard your samples after completion of project
3
Your experiment starts
Now it is up to us... We will start your experiment as soon as possible.
When expedited, the turn-around-time (TAT) is 2 weeks
For standard requests the turn-around-time (TAT) is 2 months
The standard TAT is generally overestimated, but this extended timeframe allows us to : combine similar requests of multiple scientists and to repeat the experiment in case of issues.
Please refrain from contacting us in case the pre-agreed TAT didn't expire yet.
4
Genelist
ABL1
ADA2
AIRE
ANK2
ARID1A
ARID1B
ARID2
ARPC1B
ATM
B2M
BCL2
BCL6
BCL7A
BTK
CARD11
CASP8
CASP10
CCND1
CCND2
CCND3
CD27
CD28
CD58
CD79A
CD79B
CDC27
CDK12
CDKN2A
CDKN2B
CDKN2C
CEBPA
CHEK1
CHEK2
CIITA
CREBBP (CBP)
CSF2RB
CSMD3
CTLA4
CXCR4
DDX3X
DICER1
DIS3
DNMT3A
DOCK8
DUSP2
EP300
EZH2
FANCL
FAS
FASLG
FAT3
FBXW7
FCGBP
FGFR1
FGFR2
FGFR3
FOXL2
H1-4 (HIST1H1E)
H3C2 (HIST1H3B)
H3C3 (HIST1H3C)
HRAS
HSPG2
HYDIN
ID3
IDH1
IDH2
IGLL5
IKZF1
IL4R
IRF4
IRF8
JAK2
JAK3
JUNB
KDM6B
KLF2
KMT2C
KMT2D (MLL2)
KRAS
LRBA
LRP1B
LTB
LZTR1
MAP2K2
MEF2B
MSC
MYC
MYD88
NFKB1
NFKB2
NFKBIA
NFKBIE
NOL9
NOTCH1
NOTCH2
NRAS
NSD2
NUTM1
PBRM1
PDCD1
PDGFRA
PIK3CD
PIK3R1
PIM1
PLCG1
PLCG2
PRDM1
PRF1
PRKAR1A
PTPRD
RET (MEN2)
RNF43
RPS15
RYR1
SF3B1
SGK1
SMARCA4
SOCS1
STARD9
STAT1
STAT3
STAT5B
STAT6
SYNE1
TBL1XR1
TCF3
TERC
TERT
TET2
TNFAIP3
TNFRSF13B
TNFRSF14
TP53
TPMT
TRAF3
TRRAP
UBR5
VAV1
XPO1