Massive Parallel Sequencing (MPS)

The fleet of MPS devices at BRIGHTcore comprise the Illumina NovaSeq 6000, the Illumiva HiSeq 1500 & the Illumina MiSeq. A summary of the main characteristics can be found below.

Illumina NovaSeq 6000

Illumina HiSeq 1500

Illumina MiSeq

Applications

​Whole genome sequencing (WGS), Whole exome sequencing (WES), large gene panels, RNAseq, Methyl-seq, ChIPseq 

Maximum read length

  • S1 Flow cell: 2×150 bp

  • S2 Flow cell: 2×150 bp

  • S4 Flow cell: 2×150 bp

Throughput for maximum read length

  • ​S1 Flow cell: 24h 

  • S2 Flow cell: 36h 

  • S4 Flow cell: 44h

Maximum capacity (bases)

  • ​S1 Flow cell:  500Gb

  • S2 Flow cell: 1000Gb

  • S4 Flow cell: 3000Gb

Maximum capacity (reads)

  • ​S1 Flow cell: 3.2 B

  • S2 Flow cell: 6.6 B

  • S4 Flow cell: 20 B

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Whole genome sequencing (WGS), Whole exome sequencing (WES), large gene panels, RNAseq, Methyl-seq, ChIPseq 


  • HO Flow cell (v4) : 2x125bp

  • Rapid Flow cell (v2) : 2x250bp


  • HO Flow cell (v4) : 144h (6 days)

  • Rapid Flow cell (v2) : 60h (2,5 days)


  • HO Flow cell (v4) : 450-500 Gb

  • Rapid Flow cell (v2) : 125-150 Gb


  • HO Flow cell (v4) : 2000 M

  • Rapid Flow cell (v2) : 300 M

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Small genomes, small gene panels, mitochondrial resequencing, amplicon based resequencing


  • Standard v2 : 2x250bp

  • Standard v3 : 2x300bp

  • Nano v2 : 2x250bp

  • Micro v2 : 2x150bp


  • Standard v2 : 40h

  • Standard v3 : 56h

  • Nano v2 : 28h

  • Micro v2 : 19h


  • Standard v2 : 12,5 Gb

  • Standard v3 : 15 Gb

  • Nano v2 : 0,5 Gb

  • Micro v2 : 1,2 Gb


  • Standard v2 : 25 M

  • Standard v3 : 25 M

  • Nano v2 : 1 M

  • Micro v2 : 4 M

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BRIGHTcore / T +32 (0)2 477 64 79 / F +32 (0)2 477 68 59 / © 2015 by BRIGHTcore

Photography / Bart Moens / http://www.fotobart.be

Content & illustrations / Ben Caljon