
FREQUENTLY ASKED QUESTIONS
We prefer the following buffer : 10 mM Tris-HCl pH8 + 0.1 mM EDTA
Other buffers are possible, as long as you take the test specific "Minimum requirements" into consideration.
For this, please consider the "What we do" section.
You can send your samples to the following address :
UZ Brussel - BRIGHTcore
T.a.v. Ben Caljon
Laarbeeklaan 101
1090 Brussel
Please notify BRIGHTcore up front if you're sending samples. This way we can track if your samples suffer from a delay in sample shipment.
Also, please include a letter listing all samples and the test requested, preferably by filling out our sample submission form.
That depends on the sample type and the requested assay :
DNA : can be shipped at room temperature
RNA : should be shipped on
Ice : if samples take less than 20 minutes to reach our lab
Dry ice : if sample shipment takes more than 20 minutes
Blood : EDTA blood (preferably 9 ml) is stable for 2 days at room temperature and 1 week at 2-8°C
Plasma :
For cfDNA analyses, plasma should be shipped to our lab within 2 days (at room temperature) or 1 week if frozen,
For cfRNA analyses, samples should be send on dry ice
Tissue :
If the tissue is not intended to create a cell culture, please freeze sample as soon as possible (DNA/RNA) or stabilize in RNAlater (RNA) and ship the samples on dry ice
If the tissue is intended for culturing (eg. skin biopsy), please provide us with the sample at room temperature as soon as possible
Cell culture :
For DNA assays : send culture in fresh medium or send pellet on dry ice
For RNA assays : send culture in fresh medium or stabilize in RNAlater and ship frozen
Yes - upon request - samples can be shipped back to your lab. Please notify us up front OR within 2 months following completion of your sequencing experiment.
Please indicate in the sample submission form whether or not your samples need to be returned (or not).
Standardly, samples will remain stored at our facilities during 2 months following completion of the sequencing experiment. Please note that after this period your samples will be removed from our cooled storage.
Yes, the DNA and RNA extraction can be performed at our facilities, as long as the samples are of human origin. We have expertise in extracting DNA and RNA from a wide variety of tissues, however, we currently don't perform FFPE DNA/RNA extractions at our premises.