GENE PANELS

LYMFOID MALIGNANCIES: IGH, IGK AND TCR BETA/GAMMA/DELTA REARRANGEMENTS

DETAILS

 
Version :
Status :
Size :
Website :
1
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5 genes
Extra info :

Field of application

  • Diagnosis chronic lymphoid malignancies i.e. multiple myeloma, B-NHL, T-NHL… (RIZIV/INAMI reimbursement code: 588475-588486)

  • Diagnosis acute lymphoid malignancies i.e. ALL (RIZIV/INAMI reimbursement code: 588490-588501)

  • Follow-up chronic lymphoid malignancies i.e. multiple myeloma, B-NHL, T-NHL… (RIZIV/INAMI reimbursement codes: 588571-588582)

  • Follow-up acute lymphoid malignancies i.e. ALL (RIZIV/INAMI reimbursement code: 588571-588582)

Analysis: uitvoerfrequentie 1x/week. TAT maximaal 3 weken

  • Diagnostic samples:

  1. LymphoTrack kits: LymphoTrack® Dx Software - MiSeq® - CE-IVD (Invivoscribe, 95000009)

  2. EuroClonality protocol: Vidjil platform – RUO - (Duez M, Giraud M, Herbert R, Rocher T, Salson M, Thonier F. Vidjil: A Web Platform for Analysis of High-Throughput Repertoire Sequencing. PLoS One. 2016 Nov 11;11(11):e0166126. doi: 10.1371/journal.pone.0166126. Erratum in: PLoS One. 2017 Feb 9;12 (2):e0172249. PMID: 27835690; PMCID: PMC5106020.)

  • Follow-up samples

  1. LymphoTrack® Dx Software - MiSeq® - CE-IVD (Invivoscribe, 95000009) in combination with the tracking of gBlocks (calibrators) – BELAC accreditated – (Van der Straeten J, De Brouwer W, Kabongo E, Dresse MF, Fostier K, Schots R, Van Riet I, Bakkus M. Validation of a PCR-Based Next-Generation Sequencing Approach for the Detection and Quantification of Minimal Residual Disease in Acute Lymphoblastic Leukemia and Multiple Myeloma Using gBlocks as Calibrators. J Mol Diagn. 2021 May;23(5):599-611. doi: 10.1016/j.jmoldx.2021.01.009. Epub 2021 Feb 4. PMID: 33549860.)

Method :

IGH-Fr1: LymphoTrack® Dx IGH FR1 Assay Panel - MiSeq® - CE-IVD (Invivoscribe, 91210039)

IGH-Fr3: LymphoTrack® Dx IGH FR3 Assay Panel - MiSeq® - CE-IVD (Invivoscribe, 91210119)

IGH-VHLeader: LymphoTrack® Dx IGHV SHM Assay Panel - MiSeq® - CE-IVD (Invivoscribe, 91210069)

IGK: LymphoTrack® Dx IGK Assay Panel - MiSeq® - CE-IVD (Invivoscribe, 91220019)

TCRB: LymphoTrack® Dx TRB Assay Panel - MiSeq® - CE-IVD (Invivoscribe, 92250019)

TCRG: LymphoTrack® Dx TRG Assay Panel - MiSeq® - CE-IVD (Invivoscribe, 92270009)

TCRD, IGHinc: EuroClonality protocol – RUO - (Brüggemann M, Kotrová M, Knecht H, Bartram J, Boudjogrha M, Bystry V, Fazio G, Froňková E, Giraud M, Grioni A, Hancock J, Herrmann D, Jiménez C, Krejci A, Moppett J, Reigl T, Salson M, Scheijen B, Schwarz M, Songia S, Svaton M, van Dongen JJM, Villarese P, Wakeman S, Wright G, Cazzaniga G, Davi F, García-Sanz R, Gonzalez D, Groenen PJTA, Hummel M, Macintyre EA, Stamatopoulos K, Pott C, Trka J, Darzentas N, Langerak AW; EuroClonality-NGS working group. Standardized next-generation sequencing of immunoglobulin and T-cell receptor gene recombinations for MRD marker identification in acute lymphoblastic leukaemia; a EuroClonality-NGS validation study. Leukemia. 2019 Sep;33(9):2241-2253. doi: 10.1038/s41375-019-0496-7. Epub 2019 Jun 26. PMID: 31243313; PMCID: PMC6756028)

CERTIFICATES

 
141-MED (UZ Brussel)
141-MED (UZ Brussel)

INSTRUMENTS

 
Illumina MiSeq
Illumina MiSeq

Our lowest capacity short reads sequencer, able to generate reads up to 300 bp in length and this at a throughput of 1M to 30M reads per run.

DOWNLOADS

 

CONTACT

 
pas14klein.jpg

Secretariaat Moleculaire hematologie

+32 (0)2 477 50 30 (sec)

UZ Brussel

Moleculaire hematologie

Laarbeeklaan 101

1090 Brussels

pas14klein.jpg

Marleen Bakkus

+32 (0)2 477 50 30 (sec)

UZ Brussel

Moleculaire hematologie

Laarbeeklaan 101

1090 Brussels

pas14klein.jpg

Barbara Depreter

+32 (0)2 477 50 30 (sec)

UZ Brussel

Moleculaire hematologie & HLA typing

Laarbeeklaan 101

1090 Brussels

SAMPLE TYPES

 

We aim to keep the list with sample types updated. However, if you believe we offer this gene panel on other sample types, or if you have a very specific sample type you'd like to evaluate : please contact the corresponding persons for either diagnostic or research purposes.

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Tissue
Recipient
Quantity
Transport
Purpose / Extract
Blood
EDTA tube
10 ml (min. 3 ml)
Room temperature
DNA
DNA from various tissues
Microtube*
See test details/specifications
Room temperature
DNA
DNA from various tissues
Microtube* or 96 well plate
See test details/specifications
Room temperature
DNA

* Microtube can be  a either a cryovial or eppendorf tube (0,5 ; 1,5 or 2 ml). Please consider the quality of the tube used (nuclease free ; free of RNA/DNA ; sterile ; LoBind) according to application needed.  

DELIVERABLES

 

Which data files can be retrieved from us for this specific gene panel?

Deliverable
Description
Research or diagnostic
Clinical report
Diagnostic report generated by Clinical Geneticist/ Pathologist / Hematologist
Diagnostic
 

GENELIST

IGH
IGK
TRB (TCRB)
TRG (TCRG)
TRD (TCRD)