GENE PANELS
11-LOCI HLA TYPING FOR HSC AND SOLID ORGAN TRANSPLANTATION
DETAILS
Version :
Status :
Size :
Website :
1
11 genes
Extra info :
Field of application
The human leukocyte antigen (HLA) genes are the most polymorphic genes in the human genome. The degree of HLA matching between transplant recipients and donors significantly affects the outcomes of both solid organ transplant and hematopoietic stem cell transplantation (HSCT) The implementation of NGS technologies in routine clinical work permits in-depth typing of 11 HLA genes; thereby providing optimal HLA matching of donor-recipient pairs for transplantation.
Analysis: uitvoerfrequentie 1x/week. TAT maximaal 3 weken
Method :
Holotype HLA 24/11 CE kit (Omixon)
The Holotype HLA Assay provides amplification and library preparation reagents for comprehensive gene coverage up to 11 HLA loci.
The Software, Omixon HLA Twin, features two independent algorithms for double validation by data analysis to deliver the most accurate, high-resolution genotyping available.
CERTIFICATES
141-MED (UZ Brussel)
EFI
INSTRUMENTS
Illumina MiSeq
Our lowest capacity short reads sequencer, able to generate reads up to 300 bp in length and this at a throughput of 1M to 30M reads per run.
DOWNLOADS
CONTACT
Secretariaat HLA labo
+32 (0)2 477 50 30 (sec)
UZ Brussel
HLA laboratorium
Laarbeeklaan 101
1090 Brussels
Sonja Verheyden
+32 (0)2 477 50 30 (sec)
UZ Brussel
HLA laboratorium
Laarbeeklaan 101
1090 Brussels
Barbara Depreter
+32 (0)2 477 50 30 (sec)
UZ Brussel
Moleculaire hematologie & HLA typing
Laarbeeklaan 101
1090 Brussels
SAMPLE TYPES
We aim to keep the list with sample types updated. However, if you believe we offer this gene panel on other sample types, or if you have a very specific sample type you'd like to evaluate : please contact the corresponding persons for either diagnostic or research purposes.
Tissue | Recipient | Quantity | Transport | Purpose / Extract |
|---|---|---|---|---|
Blood | EDTA tube | 10 ml (min. 3 ml) | Room temperature | DNA |
DNA from various tissues | Microtube* | See test details/specifications | Room temperature | DNA |
DNA from various tissues | Microtube* or 96 well plate | See test details/specifications | Room temperature | DNA |
* Microtube can be a either a cryovial or eppendorf tube (0,5 ; 1,5 or 2 ml). Please consider the quality of the tube used (nuclease free ; free of RNA/DNA ; sterile ; LoBind) according to application needed.
DELIVERABLES
Which data files can be retrieved from us for this specific gene panel?
Deliverable | Description | Research or diagnostic |
|---|---|---|
Clinical report | Diagnostic report generated by Clinical Geneticist/ Pathologist / Hematologist | Diagnostic |
Research report | Research report generated by Clinical Geneticist/ Pathologist / Hematologist | RUO |