GENE PANELS

11-LOCI HLA TYPING FOR HSC AND SOLID ORGAN TRANSPLANTATION

DETAILS

Panel Details

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11 genes

Extra info :

Field of application

The human leukocyte antigen (HLA) genes are the most polymorphic genes in the human genome. The degree of HLA matching between transplant recipients and donors significantly affects the outcomes of both solid organ transplant and hematopoietic stem cell transplantation (HSCT) The implementation of NGS technologies in routine clinical work permits in-depth typing of 11 HLA genes; thereby providing optimal HLA matching of donor-recipient pairs for transplantation.

Analysis: uitvoerfrequentie 1x/week. TAT maximaal 3 weken

Method :

Holotype HLA 24/11 CE kit (Omixon)
The Holotype HLA Assay provides amplification and library preparation reagents for comprehensive gene coverage up to 11 HLA loci.
The Software, Omixon HLA Twin, features two independent algorithms for double validation by data analysis to deliver the most accurate, high-resolution genotyping available.

CERTIFICATES

Certificates

141-MED (UZ Brussel)

EFI

INSTRUMENTS

Instruments

Illumina MiSeq

Our lowest capacity short reads sequencer, able to generate reads up to 300 bp in length and this at a throughput of 1M to 30M reads per run.

DOWNLOADS

Downloads

CONTACT

Contacts

Secretariaat HLA labo

labohemato@uzbrussel.be

+32 (0)2 477 50 30 (sec)

UZ Brussel

HLA laboratorium

Laarbeeklaan 101

1090 Brussels

Sonja Verheyden

sonja.verheyden@uzbrussel.be

+32 (0)2 477 50 30 (sec)

UZ Brussel

HLA laboratorium

Laarbeeklaan 101

1090 Brussels

Barbara Depreter

barbara.depreter@uzbrussel.be

+32 (0)2 477 50 30 (sec)

UZ Brussel

Moleculaire hematologie & HLA typing

Laarbeeklaan 101

1090 Brussels

SAMPLE TYPES

Sample types

We aim to keep the list with sample types updated. However, if you believe we offer this gene panel on other sample types, or if you have a very specific sample type you'd like to evaluate : please contact the corresponding persons for either diagnostic or research purposes.

Tissue	Recipient	Quantity	Transport	Purpose / Extract
Blood	EDTA tube	10 ml (min. 3 ml)	Room temperature	DNA
DNA from various tissues	Microtube*	See test details/specifications	Room temperature	DNA
DNA from various tissues	Microtube* or 96 well plate	See test details/specifications	Room temperature	DNA

* Microtube can be a either a cryovial or eppendorf tube (0,5 ; 1,5 or 2 ml). Please consider the quality of the tube used (nuclease free ; free of RNA/DNA ; sterile ; LoBind) according to application needed.

DELIVERABLES

Deliverables

Which data files can be retrieved from us for this specific gene panel?

Deliverable	Description	Research or diagnostic
Clinical report	Diagnostic report generated by Clinical Geneticist/ Pathologist / Hematologist	Diagnostic
Research report	Research report generated by Clinical Geneticist/ Pathologist / Hematologist	RUO

Genelist

GENELIST

HLA-A
HLA-B
HLA-C
HLA-DQA1
HLA-DQB1
HLA-DRB1
HLA-DPB1
HLA-DPA1
HLA-DRB3
HLA-DRB4
HLA-DRB5

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