GENE PANELS
11-LOCI HLA TYPING FOR HSC AND SOLID ORGAN TRANSPLANTATION
DETAILS
Version :
1
Status :
Size :
11 genes
Start date :
Website :
Extra info :
Field of application
The human leukocyte antigen (HLA) genes are the most polymorphic genes in the human genome. The degree of HLA matching between transplant recipients and donors significantly affects the outcomes of both solid organ transplant and hematopoietic stem cell transplantation (HSCT) The implementation of NGS technologies in routine clinical work permits in-depth typing of 11 HLA genes; thereby providing optimal HLA matching of donor-recipient pairs for transplantation.
Analysis: uitvoerfrequentie 1x/week. TAT maximaal 3 weken
Method :
Holotype HLA 24/11 CE kit (Omixon)
The Holotype HLA Assay provides amplification and library preparation reagents for comprehensive gene coverage up to 11 HLA loci.
The Software, Omixon HLA Twin, features two independent algorithms for double validation by data analysis to deliver the most accurate, high-resolution genotyping available.
CERTIFICATES
141-MED (UZ Brussel)
141-TEST (BRIGHTcore)
EFI
INSTRUMENTS
Illumina MiSeq
Our lowest capacity short reads sequencer, able to generate reads up to 300 bp in length and this at a throughput of 1M to 30M reads per run.
DOWNLOADS
CONTACT
Secretariaat HLA labo
+32 (0)2 477 50 30 (sec)
UZ Brussel
HLA laboratorium
Laarbeeklaan 101
1090 Brussels
Sonja Verheyden
+32 (0)2 477 50 30 (sec)
UZ Brussel
HLA laboratorium
Laarbeeklaan 101
1090 Brussels
SAMPLE TYPES
We aim to keep the list with sample types updated. However, if you believe we offer this gene panel on other sample types, or if you have a very specific sample type you'd like to evaluate : please contact the corresponding persons for either diagnostic or research purposes.
* Microtube can be a either a cryovial or eppendorf tube (0,5 ; 1,5 or 2 ml). Please consider the quality of the tube used (nuclease free ; free of RNA/DNA ; sterile ; LoBind) according to application needed.
Research portfolio
Blood > DNA
EDTA tube
10 ml (min. 3 ml)
Room temperature
DNA from various tissues > DNA
Microtube* or 96 well plate
See test details/specifications
Room temperature
Diagnostic portfolio
Blood > DNA
EDTA tube
10 ml (min. 3 ml)
Room temperature
DNA from various tissues > DNA
Microtube* or 96 well plate
See test details/specifications
Room temperature
Specific sample recommendations / requirements
No additional requirements / recommendations
Optimum : [Acceptable range (-)]
There are no specific recommendations in addition to those listed under the Sample Types
Absorbance ratio A260/A230 - Minimum
Optimum : 1,5 [Acceptable range (-)]
DNA from various tissues
Lower values are indicative for indicative for EDTA, carbohydrate, chaotropic salts (and also protein) contamination
Absorbance ratio A260/A280 - Minimum
Optimum : 1,5 [Acceptable range (-)]
DNA from various tissues
Lower values are indicative for indicative for protein, phenol or other aromatic compound contamination
DELIVERABLES
Which data files can be retrieved from us for this specific gene panel?
Deliverable | Description | Research or diagnostic |
|---|---|---|
Clinical report | Diagnostic report generated by Clinical Geneticist/ Pathologist / Hematologist | Diagnostic |
Research report | Research report generated by Clinical Geneticist/ Pathologist / Hematologist | RUO |
PLAN EXPERIMENT
Please follow the steps below to start up an experiment with us.
Get in touch (ONLY FOR NEW PROJECTS)
Explain your project, so we can assist you to find the best solution.
Expect questions like :
What type of sample would you like to analyze?
Which elution buffer did you use?
What are the expected concentrations of your samples?
How many samples would you like to process?
How fast do you need the results (TAT) and/or do we need to expedite?
If you deviate from our sample types, are there test samples available?
Are our standard deliverables ok?
...
1
Request quotation
Once you know which experiment you want to set up, fill out the 'Quote request' form, with the essential details of your project :
Coordinates of the person to whom to address the quotation
Which type of test you'd like to set up
Do you require a specific deliverable?
Do we need to expedite (comes at extra cost)?
...
2
Submit your samples
Once you agree with our terms (quote / TAT / terms & conditions) :
Fill out our sample submission form
Send it via email : contact us
Print out the form and include it with your samples
Send your samples to us
Follow our device dependent instructions : see details here
Label your tubes/plates correctly : see details here
Adhere to the corresponding transport conditions listed under Sample types
Indicate if we can discard your samples after completion of project
3
Your experiment starts
Now it is up to us... We will start your experiment as soon as possible.
When expedited, the turn-around-time (TAT) is 2 workdays
For standard requests the turn-around-time (TAT) is 5 workdays
The standard TAT is generally overestimated, but this extended timeframe allows us to : combine similar requests of multiple scientists and to repeat the experiment in case of issues.
Please refrain from contacting us in case the pre-agreed TAT didn't expire yet.
