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GENE PANELS

EXOME - MALE INFERTILITY (MINF)

Engineering Plans

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Version :

1

Status :
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Size :

93 genes

Start date :

January 22, 2025

Website :

Extra info :

MINFGP

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Sample types

We aim to keep the list with sample types updated. However, if you believe we offer this gene panel on other sample types, or if you have a very specific sample type you'd like to evaluate : please contact the corresponding persons for either diagnostic or research purposes.

* Microtube can be  a either a cryovial or eppendorf tube (0,5 ; 1,5 or 2 ml). Please consider the quality of the tube used (nuclease free ; free of RNA/DNA ; sterile ; LoBind) according to application needed.  

Blood > DNA

DBS / Guthrie card / Punch in microtube*

Min. 1 punch

Room temperature

Blood > DNA

EDTA tube

10 ml (min. 3 ml)

Room temperature

Cells of various tissue origins > DNA

Microtube* with cell pellet or on lysis buffer

> 5 million cells

Dry ice

DNA from various tissues > DNA

Microtube* or 96 well plate

See test details/specifications

Room temperature

Tissue of various origins > DNA

Cryovial containing stabilizer (eg. AllProtect) or tissue homogenized on lysis buffer

Application/tissue specific (contact us)

Dry ice

Extra requirements

Absorbance ratio A260/A230 - Minimum
DNA from various tissues

Minimum: 1,5

Lower values are indicative for indicative for EDTA, carbohydrate, chaotropic salts (and also protein) contamination

Absorbance ratio A260/A280 - Minimum
DNA from various tissues

Minimum: 1,5

Lower values are indicative for indicative for protein, phenol or other aromatic compound contamination

Elution buffer : nuclease free water, Tris-HCl or TE
DNA from various tissues

:

Please use nuclease free elution buffers, however this assay is compatible with either water, Tris-HCl as TE.

Minimum DNA concentration
DNA from various tissues

Minimum: 7,7 ng/µl

A minimum concentration of 7,7 ng/µl is required, however succesfull experiments are possible below this limit.

Minimum DNA quantity
DNA from various tissues

Minimum: 200 ng

We prefer delivery of at least 1 µg of DNA

Image by Mezidi Zineb
Sample types
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Deliverables

Below, you can find the type of data files that can be retrieved for this test.

If you require more information or need a more custom output, please consult our Bioinformatics page.

Called variants - vcf file

Text file containing all detected variants as compared to the relevant reference genome

Diagnostic / RUO
Clinical report

Diagnostic report generated by Clinical Geneticist/ Pathologist / Hematologist

Diagnostic
Raw data - bam file

Binary data file containing the aligned reads

RUO (on request)
Raw data - fastq file

Data file containing the raw (unaligned) reads

RUO (on request)
Run folder

The complete run folder can be transferred if it concerns a run reservation (private run)

RUO (on request)
Deliverables

Costs

For Diagnostic testing

When requested through your General Practitioner / Clinical Geneticist / Pathologist / Hematologist, costs will be covered through your health insurance. The costs involved will be those for your consultation and/or 'remgeld'.

For Research testing

Below, you can find indicative prices (excl. VAT) for all potential options you'd require to accomplish your project. If you are interested, please contact us to receive a tailored quotation.

Update pending

Our prices are being updated. More info soon.

Euro Bill
Costs
Documents
Books

Documents & certificates

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Validated
Plan experiment

Plan experiment

  1. For Diagnostics

This test is mainly performed as a diagnostic assay. In this case, please contact your General Practitioner or Clinical Geneticist (Centre for Medical Genetics of choice) to get the diagnosis started.


  1. For Research

This test can also be applied in context of research. For this, please get approval from your local ethical committee and/or make proper arrangements with your Biobank.

Please follow the steps below to start up an experiment with us.

Get in touch (ONLY FOR NEW PROJECTS)

Explain your project, so we can assist you to find the best solution.

Expect questions like :

  1. What type of sample would you like to analyze?

  2. Which elution buffer did you use?

  3. What are the expected concentrations of your samples?

  4. How many samples would you like to process?

  5. How fast do you need the results (TAT) and/or do we need to expedite?

  6. If you deviate from our sample types, are there test samples available?

  7. Are our standard deliverables ok?

  8. ...

Call Center Headset

1

Request quotation

Once you know which experiment you want to set up, fill out the 'Quote request' form, with the essential details of your project :

  1. Coordinates of the person to whom to address the quotation

  2. Which type of test you'd like to set up

  3. Do you require a specific deliverable?

  4. Do we need to expedite (comes at extra cost)?

  5. ...

Call Center Headset

2

Submit your samples

Once you agree with our terms (quote / TAT / terms & conditions)  :

  1. Fill out our sample submission form

  2. Send it via email : contact us

  3. Print out the form and include it with your samples

  4. Send your samples to us

  5. Label your tubes/plates correctly : see details here

  6. Adhere to the corresponding transport conditions listed under Sample types

  7. Indicate if we can discard your samples after completion of project

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3

Your experiment starts

Now it is up to us... We will start your experiment as soon as possible.

  1. When expedited, the turn-around-time (TAT) is 1 month

  2. For standard requests the turn-around-time (TAT) is 2 months


The standard TAT is generally overestimated, but this extended timeframe allows us to : combine similar requests of multiple scientists and to repeat the experiment in case of issues.


Please refrain from contacting us in case the pre-agreed TAT didn't expire yet.

Call Center Headset

4

Genelist
White Rocks

Genelist

ACTL9
ADGRG2
APOA1
AR
ARMC2
AURKC
C14orf39
SHOC1
CATIP
CATSPER1
CATSPER2
KASH5
CCDC39
CCDC40
CDC14A
CFAP43
CFAP44
CFAP58
CFAP65
CFAP69
CFTR
DMC1
DMRT1
DNAAF2
DNAAF4
DNAAF5
DNAH1
DNAH10
DNAH17
DNAH7
DNAH8
DNAI1
DNAI2
DNAJB13
DNHD1
DPY19L2
DRC1
FANCA
FANCM
FKBP6
FSHR
FSIP2
GAS8
GCNA
HSF2
KLHL10
LHCGR
DNAAF11
M1AP
CFAP91
MCM8
MCM9
MEI1
MEIOB
MLH3
MNS1
MSH4
MSH5
NR5A1
PDHA2
DNAAF6
PMFBP1
PNLDC1
QRICH2
RNF212
RSPH3
RSPH9
SEPTIN12
SPAG6
SPATA16
SPATA22
SPEF2
SPINK2
SPO11
STAG3
SUN5
SYCE1
SYCP2
SYCP3
TAF4B
TDRD9
TERB1
TERB2
TEX11
TEX14
TEX15
TSGA10
TTC29
USP26
USP9Y
CFAP251
XRCC2
ZMYND15

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BRIGHTcore / T +32 (0)2 477 64 79 / F +32 (0)2 477 68 59

Photography / See our Colofon

Content & illustrations / Ben Caljon

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