GENE PANELS
SOLID TUMORS AND HAEMATOLOGICAL TUMORS (STHT)
DETAILS
CERTIFICATES
141-MED (UZ Brussel)
INSTRUMENTS
Illumina NovaSeq 6000
Our highest capacity short read sequencer. This device can generate reads up to 250 bp in length (on the SP flowcell) and generate up to 10B reads per flowcell (S4).
DOWNLOADS
Genelist STHT
2
STHT subpanels overview
2
Verzendinstructies DNA stalen - NL
1
CONTACT
BRIGHTcore general inquires
+32 (0)2 477 64 79 (sec)
UZ Brussel
BRIGHTcore
Laarbeeklaan 101
1090 Brussels
SAMPLE TYPES
We aim to keep the list with sample types updated. However, if you believe we offer this gene panel on other sample types, or if you have a very specific sample type you'd like to evaluate : please contact the corresponding persons for either diagnostic or research purposes.
Tissue | Recipient | Quantity | Transport | Purpose / Extract |
|---|---|---|---|---|
Blood | EDTA tube | 10 ml (min. 3 ml) | Room temperature | DNA |
DNA from various tissues | Microtube* | See test details/specifications | Room temperature | DNA |
DNA from various tissues | Microtube* or 96 well plate | See test details/specifications | Room temperature | DNA |
FFPE block of various tissues | Paraffin block (in cassette) | Representative block ; Tumor load preferentially >10% ; To be processed by our pathology department (microtome section needed) | Room temperature | DNA |
FFPE section of various tissues | Microtube* | Min. 1x 10 µm section (up to 2 mm³ tissue) ; Tumor load preferentially >10% | Room temperature | DNA |
Tissue of various origins | Cryovial containing stabilizer (eg. AllProtect) or tissue homogenized on lysis buffer | Application/tissue specific (contact us) | Dry ice | DNA |
* Microtube can be a either a cryovial or eppendorf tube (0,5 ; 1,5 or 2 ml). Please consider the quality of the tube used (nuclease free ; free of RNA/DNA ; sterile ; LoBind) according to application needed.
DELIVERABLES
Which data files can be retrieved from us for this specific gene panel?
Deliverable | Description | Research or diagnostic |
|---|---|---|
Called variants - vcf file | Text file containing all detected variants as compared to the relevant reference genome | Diagnostic / RUO |
Clinical report | Diagnostic report generated by Clinical Geneticist/ Pathologist / Hematologist | Diagnostic |
Raw data - bam file | Binary data file containing the aligned reads | RUO (on request) |
Raw data - fastq file | Data file containing the raw (unaligned) reads | RUO (on request) |
Run folder | The complete run folder can be transferred if it concerns a run reservation (private run) | RUO (on request) |
GENELIST
ABL1
ACVR1
AKT1
ALK
APC
AR
ARAF
ARID1A
ASXL1
ATM
ATR
ATRX
AXIN1
BAP1
BARD1
BCL2
BCL6
BCOR
BRAF
BRCA1
BRCA2
BRIP1
BTK
CALR
CARD11
CBL
CCND1
CD79B
CDH1
CDK12
CDKN2A
CDKN2B
CDKN2C
CEBPA
CHEK1
CHEK2
CIC
CRBN
CREBBP
CSF3R
CTNNB1
CUL4B
CXCR4
CYLD
DAXX
DDR2
DICER1
DIS3
DNMT3A
EGFR
EGR1
EIF1AX
EP300
EPCAM
ERBB2
ERBB3
ERBB4
ESR1
ETV6
EZH2
FAM175A
FAM46C
FANCA
FANCL
FAU
FBXW7
FGFR1
FGFR2
FGFR3
FLT3
FOXL2
FOXO1
FUBP1
GNA11
GNAQ
GNAS
H3F3A
H3F3B
HIST1H1E
HIST1H3B
HIST1H3C
HRAS
IDH1
IDH2
IKZF1
IRF4
JAK2
JAK3
KIT
KMT2A
KMT2D
KRAS
LTB
MAP2K1
MAP2K2
MEF2B
MET
MLH1
MPL
MRE11
MSH2
MSH6
MTOR
MUTYH
MYD88
MYOD1
NBN
NF1
NF2
NOTCH1
NPM1
NRAS
NTRK1
NTRK2
NTRK3
NUTM1
PALB2
PAX8
PDGFRA
PDGFRB
PIK3CA
PIK3R1
PMS2
POLD1
POLE
PPM1D
PRKAR1A
PTEN
PTPN11
RAD50
RAD51B
RAD51C
RAD51D
RAD54L
RASAL1
RB1
RET
RHOA
RICTOR
ROS1
RUNX1
SETBP1
SF3B1
SMAD4
SMARCA4
SMARCB1
SMO
SRSF2
STAG2
STAT3
STK11
TERT
TET2
TNFAIP3
TNFRSF14
TP53
TRAF3
TSC1
TSC2
U2AF1
VAV1
VHL
WT1
ZRSR2
XRCC2