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RNA sequencing

Engineering Plans

Learn more

Learn more

Specifications & applications

Our highest capacity short read sequencer. This device can generate reads up to 250 bp in length (on the SP flowcell) and generate up to 10B reads per flowcell (S4).
Illumina NovaSeq 6000
Flow cell types

SP
S1
S2
S4

Max. read length

250 bp
150 bp
150 bp
150 bp

Max. run time

38h
25h
36h
44h

Max. capacity (bases)

400 Gb
500 Gb
1250 Gb
3000 Gb

Max. capacity (reads)*

0,8B SE - 1,6B PE
1,6B SE - 3,2B PE
4,1B SE - 8,2B PE
10B SE - 20B PE

Image by Lukas Tennie
Specifications
Image by Mezidi Zineb

Sample types

Blood > RNA

PAXgene Blood RNA tube

10 ml (2,5 ml blood)

Ice pack

Blood > cfRNA/exoRNA

Streck RNA Complete BCT

10 ml

Room temperature

Cells of various tissue origins > RNA

Microtube* with cells on lysis buffer

> 5 million cells

Dry ice

FFPE block of various tissues > RNA

Paraffin block (in cassette)

Representative block ; Tumor load preferentially >10% ; To be processed by our pathology department (microtome section needed)

Room temperature

FFPE section of various tissues > RNA

Microtube*

Min. 1x 10 µm section (up to 2 mm³ tissue) ; Tumor load preferentially >10%

Room temperature

Plasma > cfRNA/exoRNA

DNA LoBind 5 ml tube

> 2ml

Dry ice

RNA from various tissues > RNA

Microtube*

See test details/specifications

Ice (max. 20 min) or dry ice

RNA from various tissues > RNA

Microtube* or 96 well plate

See test details/specifications

Ice (max. 20 min) or dry ice

Tissue of various origins > RNA

Cryovial containing stabilizer (eg. RNAlater) or tissue homogenized on lysis buffer

Application/tissue specific (contact us)

Dry ice

Extra requirements

Sample types

Deliverables

Below, you can find the type of data files that can be retrieved for this test.

If you require more information or need a more custom output, please consult our Bioinformatics page.

Run folder

The complete run folder can be transferred if it concerns a run reservation (private run)

RUO (on request)
Raw data - fast5 file

Raw data file generated by Oxford NanoPore sequencers

RUO (on request)
Raw data - bam file

Binary data file containing the aligned reads

RUO (on request)
Raw data - fastq file

Data file containing the raw (unaligned) reads

RUO (on request)
Cardboard Box
Deliverables
Euro Bill

Costs

Below, you can find indicative prices (excl. VAT) for all potential options you'd require to accomplish your project. If you are interested, please contact us to receive a tailored quotation.

Costs

Documents & certificates

Under validation
Under validation
Books
Documents
Plan Experiment

Plan experiment

Related tests

Related tests
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BRIGHTcore / T +32 (0)2 477 64 79 / F +32 (0)2 477 68 59

Photography / See our Colofon

Content & illustrations / Ben Caljon

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