RNA sequencing

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The instrument(s) listed below are relevant in context of this test :

Illumina NovaSeq 6000

Our highest capacity short read sequencer. This device can generate reads up to 250 bp in length (on the SP flowcell) and generate up to 10B reads per flowcell (S4).

MGI DNBSEQ-T7

Our highest capacity short read sequencer. This device can generate up to 5,8B reads per flowcell. We standardly run SE50 bp and PE150 bp runs, but read length can be flexibily adjusted (to >200 bp).

Learn more

Specifications & applications

Our highest capacity short read sequencer. This device can generate up to 5,8B reads per flowcell. We standardly run SE50 bp and PE150 bp runs, but read length can be flexibily adjusted (to >200 bp).

MGI DNBSEQ-T7

Our highest capacity short read sequencer. This device can generate reads up to 250 bp in length (on the SP flowcell) and generate up to 10B reads per flowcell (S4).

Illumina NovaSeq 6000

Flow cell types

v3 (SM1.0)
v5 (SM2.0)

SP
S1
S2
S4

Max. read length

v3 (SM1.0) : 150 bp
v5 (SM2.0) : 150 bp

SP : 250 bp
S1 : 150 bp
S2 : 150 bp
S4 : 150 bp

Max. run time

v3 (SM1.0) : 24-30h
v5 (SM2.0) : 24-30h

SP : 38h
S1 : 25h
S2 : 36h
S4 : 44h

Max. capacity (bases)

v3 (SM1.0) : 1500 Gb
v5 (SM2.0) : 1500 Gb

SP : 400 Gb
S1 : 500 Gb
S2 : 1250 Gb
S4 : 3000 Gb

Max. capacity (reads)*

v3 (SM1.0) : 5,8B SE - 11,6 PE
v5 (SM2.0) : 5,8B SE - 11,6 PE

SP : 0,8B SE - 1,6B PE
S1 : 1,6B SE - 3,2B PE
S2 : 4,1B SE - 8,2B PE
S4 : 10B SE - 20B PE

MGI DNBSEQ-T7

Illumina NovaSeq 6000

Applications (possible)

Whole Genome Sequencing, Whole Exome Sequencing, Large Gene Panels, RNAseq, Methyl-seq, ChIPseq, Small genomes, Mitochondrial resequencing

Applications (in use)

Whole Genome Sequencing, Whole Exome Sequencing, Large Gene Panels, RNAseq, Methyl-seq, ChIPseq, Small genomes, Mitochondrial resequencing

Remarks

As the highest capacity short read instrument in our portfolio, it is the cheapest/base. Whole Human Genomes at 30x coverage are possible at prices around 500€. The system is highly flexible in run configuration and can sequence 'dark bases' on demand (eg. to circumvent unwanted polyA tails or tags/primersequences introduced during library prep).
The DNBSEQ-T7 can sequence 4 flow cells simultaneously.

As the highest capacity short read instrument from Illumina in our portfolio, it is the cheapest/base. Whole Human Genomes at 30x coverage are possible at prices around 1000€ (S4 flow cell). It's ability to sequence 250bp reads (SP flow cell) is also a big plus.

The NovaSeq can sequence 2 flow cells simultaneously.

Specifications

Sample types

Blood > RNA

PAXgene Blood RNA tube

10 ml (2,5 ml blood)

Ice pack

Blood > cfRNA/exoRNA

Streck RNA Complete BCT

10 ml

Room temperature

Cells of various tissue origins > RNA

Microtube* with cells on lysis buffer

> 5 million cells

Dry ice

FFPE block of various tissues > RNA

Paraffin block (in cassette)

Representative block ; Tumor load preferentially >10% ; To be processed by our pathology department (microtome section needed)

Room temperature

FFPE section of various tissues > RNA

Microtube*

Min. 1x 10 µm section (up to 2 mm³ tissue) ; Tumor load preferentially >10%

Room temperature

Plasma > cfRNA/exoRNA

DNA LoBind 5 ml tube

> 2ml

Dry ice

RNA from various tissues > RNA

Microtube*

See test details/specifications

Ice (max. 20 min) or dry ice

RNA from various tissues > RNA

Microtube* or 96 well plate

See test details/specifications

Ice (max. 20 min) or dry ice

Tissue of various origins > RNA

Cryovial containing stabilizer (eg. RNAlater) or tissue homogenized on lysis buffer

Application/tissue specific (contact us)

Dry ice

Blood > RNA

PAXgene Blood RNA tube

10 ml (2,5 ml blood)

Ice pack

Blood > cfRNA/exoRNA

Streck RNA Complete BCT

10 ml

Room temperature

Cells of various tissue origins > RNA

Microtube* with cells on lysis buffer

> 5 million cells

Dry ice

FFPE block of various tissues > RNA

Paraffin block (in cassette)

Representative block ; Tumor load preferentially >10% ; To be processed by our pathology department (microtome section needed)

Room temperature

FFPE section of various tissues > RNA

Microtube*

Min. 1x 10 µm section (up to 2 mm³ tissue) ; Tumor load preferentially >10%

Room temperature

Plasma > cfRNA/exoRNA

DNA LoBind 5 ml tube

> 2ml

Dry ice

RNA from various tissues > RNA

Microtube*

See test details/specifications

Ice (max. 20 min) or dry ice

RNA from various tissues > RNA

Microtube* or 96 well plate

See test details/specifications

Ice (max. 20 min) or dry ice

Tissue of various origins > RNA

Cryovial containing stabilizer (eg. RNAlater) or tissue homogenized on lysis buffer

Application/tissue specific (contact us)

Dry ice

Extra requirements

Sample types

Deliverables

Below, you can find the type of data files that can be retrieved for this test.

If you require more information or need a more custom output, please consult our Bioinformatics page.

Bioinformatics

Run folder

The complete run folder can be transferred if it concerns a run reservation (private run)

RUO (on request)

Raw data - fast5 file

Raw data file generated by Oxford NanoPore sequencers

RUO (on request)

Raw data - bam file

Binary data file containing the aligned reads

RUO (on request)

Raw data - fastq file

Data file containing the raw (unaligned) reads

RUO (on request)

Deliverables

Costs

Below, you can find indicative prices (excl. VAT) for all potential options you'd require to accomplish your project. If you are interested, please contact us to receive a tailored quotation.

Request quote

Costs

Documents & certificates

Under validation

Documents

Plan Experiment

RNA sequencing

Learn more

Specifications & applications

MGI DNBSEQ-T7

Illumina NovaSeq 6000

Flow cell types

Max. read length

Max. run time

Max. capacity (bases)

Max. capacity (reads)*

​

Sample types

Blood > RNA

Blood > cfRNA/exoRNA

Cells of various tissue origins > RNA

FFPE block of various tissues > RNA

FFPE section of various tissues > RNA

Plasma > cfRNA/exoRNA

RNA from various tissues > RNA

RNA from various tissues > RNA

Tissue of various origins > RNA

Extra requirements

Deliverables

Run folder

RUO (on request)

Raw data - fast5 file

RUO (on request)

Raw data - bam file

RUO (on request)

Raw data - fastq file

RUO (on request)

Costs

​

Documents & certificates

Under validation

Plan experiment

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