Sars-Cov2 sequencing

Learn more

Specifications & applications

Sequencing long reads is no hassle on this device. It can sequence 5 MinION flowcells simultaneously and perform the data analysis on board.

NanoPore GridION x5

Flow cell types

R9.4.1
R10.4

Max. read length

> 4 Mb

Max. run time

72h (interruptible at all time)

Max. capacity (bases)

< 50 Gb

Max. capacity (reads)*

< 5M (for avg. 10K bp reads)

NanoPore GridION x5

Applications (possible)

Small genomes, Long Range Amplicon sequencing, RNAseq, Methyl-seq, Whole Genome Sequencing

Applications (in use)

Small genomes, Long Range Amplicon sequencing, RNAseq, Methyl-seq, Whole Genome Sequencing

Remarks

The ability to generate long reads and the flexibility of the run times are highly useful in (small) genome sequencing and metagenomics. This, despite its higher error rate. Single contig small genomes are possible. Also, due to the short run times, the Nanopore technology proved to be essential in Sars-Cov2 typing.

The GridION can sequence 5 flow cells simultaneously.

Specifications

Sample types

Sars-Cov2 RNA (naso/oropharyngeal) > RNA

Microtube* or 96 wel plate (plate is preferred)

Log(copies/ml) > 3 (min. 10 µl)

Ice (max. 20 min) or dry ice

Sars-Cov2 on UTM (naso/oropharyngeal) > RNA

UTM (Universal Transport Medium) or PBS tube w/o inactivation buffer

500 µl (Ct<=25)

Room temperature

Extra requirements

Sample types

Deliverables

Below, you can find the type of data files that can be retrieved for this test.

If you require more information or need a more custom output, please consult our Bioinformatics page.

Bioinformatics

Annotated variant file

Text file containing all detected variants + relevant annotation (cross reference to various databases)

Diagnostic / RUO

Deliverables

Costs

Below, you can find indicative prices (excl. VAT) for all potential options you'd require to accomplish your project. If you are interested, please contact us to receive a tailored quotation.

Request quote

Costs

Documents & certificates

Sciensano recognized

Documents

Plan Experiment