Single-cell applications

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The instrument(s) listed below are relevant in context of this test :

10x Genomics Chromium X

Microdroplet generator to partition single cells and to barcode RNA/DNA using the 10x Genomics Next GEM technology.

Illumina NovaSeq 6000

Our highest capacity short read sequencer. This device can generate reads up to 250 bp in length (on the SP flowcell) and generate up to 10B reads per flowcell (S4).

MGI DNBelab C-TaiM4

Microdroplet generator to partition single cells or nuclei to barcode RNA. DNA/ATAC applications are currenty our of scope.

10x Genomics Controller

Microdroplet generator to partition single cells and to barcode RNA/DNA using the 10x Genomics Next GEM technology.

MGI DNBSEQ-T7

Our highest capacity short read sequencer. This device can generate up to 5,8B reads per flowcell. We standardly run SE50 bp and PE150 bp runs, but read length can be flexibily adjusted (to >200 bp).

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Specifications & applications

Microdroplet generator to partition single cells and to barcode RNA/DNA using the 10x Genomics Next GEM technology.

10x Genomics Chromium X

10x Genomics Controller

Our highest capacity short read sequencer. This device can generate up to 5,8B reads per flowcell. We standardly run SE50 bp and PE150 bp runs, but read length can be flexibily adjusted (to >200 bp).

MGI DNBSEQ-T7

v3 (SM1.0)
v5 (SM2.0)

v3 (SM1.0) : 150 bp
v5 (SM2.0) : 150 bp

v3 (SM1.0) : 24-30h
v5 (SM2.0) : 24-30h

v3 (SM1.0) : 1500 Gb
v5 (SM2.0) : 1500 Gb

v3 (SM1.0) : 5,8B SE - 11,6 PE
v5 (SM2.0) : 5,8B SE - 11,6 PE

10x Genomics Chromium X

10x Genomics Controller

MGI DNBSEQ-T7

Applications (possible)

Whole Genome Sequencing, Whole Exome Sequencing, Large Gene Panels, RNAseq, Methyl-seq, ChIPseq, Small genomes, Mitochondrial resequencing

Applications (in use)

Whole Genome Sequencing, Whole Exome Sequencing, Large Gene Panels, RNAseq, Methyl-seq, ChIPseq, Small genomes, Mitochondrial resequencing

Remarks

As the highest capacity short read instrument in our portfolio, it is the cheapest/base. Whole Human Genomes at 30x coverage are possible at prices around 500€. The system is highly flexible in run configuration and can sequence 'dark bases' on demand (eg. to circumvent unwanted polyA tails or tags/primersequences introduced during library prep).
The DNBSEQ-T7 can sequence 4 flow cells simultaneously.

Specifications

Sample types

Cells of various tissue origins > DNA

Microtube* with cell pellet or on lysis buffer

> 5 million cells

Dry ice

Cells of various tissue origins > RNA

Microtube* with cells on lysis buffer

> 5 million cells

Dry ice

Extra requirements

Sample types

Deliverables

Below, you can find the type of data files that can be retrieved for this test.

If you require more information or need a more custom output, please consult our Bioinformatics page.

Bioinformatics

Run folder

The complete run folder can be transferred if it concerns a run reservation (private run)

RUO (on request)

Raw data - fast5 file

Raw data file generated by Oxford NanoPore sequencers

RUO (on request)

Raw data - bam file

Binary data file containing the aligned reads

RUO (on request)

Raw data - fastq file

Data file containing the raw (unaligned) reads

RUO (on request)

Deliverables

Costs

Below, you can find indicative prices (excl. VAT) for all potential options you'd require to accomplish your project. If you are interested, please contact us to receive a tailored quotation.

Request quote

Costs

Documents & certificates

Under validation

Documents

Plan Experiment