The instrument(s) listed below are relevant in context of this test :
10x Genomics Chromium X
Microdroplet generator to partition single cells and to barcode RNA/DNA using the 10x Genomics Next GEM technology.
AATI Fragment Analyzer
Capillary electrophoresis device that enables qualification and quantification of (genomic) DNA samples, but also RNA samples.
Bionano Stratys
This device enables Optical Genome Mapping (OGM) of up to 12 samples (1 sample/chip) simultaneously.
Covaris M220
Low throughput DNA fragmentation device (single tube). High quality fragmentation results, but costly tubes required.
Formulatrix Mantis
Automated liquid handler, requiring virtually no plastics/tips. It dispenses (incrementally) droplets of 0,1 or 0,5 µl for the Low volume chip and 1 or 5 µl droplets for the High volume chip. It is solely used to pipette reagents (virtually no 'dead volume') and not biological samples.
Illumina MiSeq
Our lowest capacity short reads sequencer, able to generate reads up to 300 bp in length and this at a throughput of 1M to 30M reads per run.
10x Genomics Controller
Microdroplet generator to partition single cells and to barcode RNA/DNA using the 10x Genomics Next GEM technology.
Affymetrix GeneChip 3000
Medium throughput SNP array device, where 48 samples (1 sample/genechip) can be processed simultaneously per run.
Caliper LabChip GX Touch
Microchip electrophoresis device that enables speedy qualification of DNA samples of up to 6Kb in size.
Diagenode BioRuptor Pico
Medium throughput (6 - 12 samples) DNA fragmentation device resulting in high quality fragmentation results using low cost plastics.
Gilson Platemaster P220
Manual 96-channel air-displacement pipette from Gilson to manipulate large sample quantities in 96 or 384-well plates. As it is a manual pipette, it is highly versatile in usage in lab.
Illumina NovaSeq 6000
Our highest capacity short read sequencer. This device can generate reads up to 250 bp in length (on the SP flowcell) and generate up to 10B reads per flowcell (S4).
The kit(s) listed below are relevant in context of this test :
Learn more
Specifications & applications
Specifications
Applications
AATI Fragment Analyzer
NanoPore GridION x5
Caliper LabChip GX Touch
R10.4 R9.4.1 (discontinued)
> 4 Mb
72h (interruptible at all time)
< 50 Gb
< 5M (for avg. 10K bp reads)
AATI Fragment Analyzer
NanoPore GridION x5
Caliper LabChip GX Touch
Applications (possible)
DNA/NGS fragment analysis, Genomic DNA analysis, Large Fragment analysis, RNA analysis, RQN/RIN calculation, GQN calculation, DV200 calculation, Single stranded DNA (ssDNA) analysis, Cas9 mRNA polyadenylation QC, Estimate CRISPR homology-directed repair (HDR) efficiency
Small genomes, Long Range Amplicon sequencing, RNAseq, Methyl-seq, Whole Genome Sequencing
DNA/NGS fragment analysis, Genomic DNA analysis, Large Fragment analysis, RNA analysis, RQN/RIN calculation, GQN calculation, DV200 calculation, Estimate CRISPR homology-directed repair (HDR) efficiency
Applications (in use)
DNA/NGS fragment analysis, Genomic DNA analysis, Large Fragment analysis, RNA analysis, RQN/RIN calculation, DV200 calculation
Small genomes, Long Range Amplicon sequencing, RNAseq, Methyl-seq, Whole Genome Sequencing
DNA/NGS fragment analysis
Remarks
The Fragment Analyzer is a versatile device allowing for 2 simultaneous assays to run (e.g. an RNA and NGS assay) with a superior sensitivity or limit of detection. This device uses only 2 µl of the native sample to be diluted with 'Diluent marker' solution. Using a 12-capillary array, the run-time per sample is quite high. A total of 3 plates (96 well only) can be loaded simultaneously. The data analyis software is less convenient as compared to the LabChip software.
The ability to generate long reads and the flexibility of the run times are highly useful in (small) genome sequencing and metagenomics. This, despite its higher error rate. Single contig small genomes are possible. Also, due to the short run times, the Nanopore technology proved to be essential in Sars-Cov2 typing.
The GridION can sequence 5 flow cells simultaneously.
The LabChip is unmatched for its speed and ability to work with the native sample (no mixing of sample with 'diluent marker' needed) from either a 96 well or 384 well plate. However, as the device needs a volume of (minimally) 25 µl, we mostly need to dilute the sample with nuclease free water beforehand. As this device can not easily switch between different assays, current assays are limited to DNA-based assays.
Specifications
Sample types
Research
Diagnostic
Extra requirements
Sample types
Deliverables
Below, you can find the type of data files that can be retrieved for this test.
If you require more information or need a more custom output, please consult our Bioinformatics page.
Below, you can find indicative prices (excl. VAT) for all potential options you'd require to accomplish your project. If you are interested, please contact us to receive a tailored quotation.
